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re: st: multiple analysis using ANOVA?

From   David Airey <>
Subject   re: st: multiple analysis using ANOVA?
Date   Thu, 25 Sep 2003 13:45:14 -0500

First off excuse my ignorance as I'm an engineer attempting to do some
statistical analysis on gene expression data I've genereated.
Basically I have 5 genes of interest (3 candidate and 2 housekeeping) and 3
Blots which I have created myself (Triplicate). Each blot has cDNA printed
on it from samples as follows: 4 time points, two groups at each time
point: control and experimental, with 6 animals per group. From this i
generate ratios of each candidate gene to each housekeeping gene for each
animal over time.
I am looking to do a multiple analysis using a two-way Anova to determine if
at any particular time point if a particular gene is elevated at a
particular time point.
Has anyone managed to get the Neumann Keuls test running in Stata?
Sorry if i've confused anyone,
Thanks in advance,
David Hardiman
I do not think the Newman-Keuls multiple comparisons test is implemented in Stata. I couldn't find anything. On the other hand, most textbooks I've read suggest the method does not control the experiment-wise error to 0.05 as claimed. SAS, SPSS, and Minitab offer one click access to multiple comparison tests of all kinds; whether you should use a particular one in certain situations over others is another problem. There is a nice chapter on multiple comparisons in the new version of Maxwell and Delaney's text which speaks to this. You can combine anovatest with multiple test corrections in Stata 8, but that's about it, other than coding what you need directly from the data and what anova leaves behind.


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